P4ha2 ELISA Kit for Mouse(Mus musculus) | EIAab.comP4ha2 ELISA Kit for Mouse(Mus musculus) | EIAab.com

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P4ha2 (Gene name),

Prolyl 4-hydroxylase subunit alpha-2 (Protein name ), P4HA2_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

P4ha2;

Protein name:

Prolyl 4-hydroxylase subunit alpha-2(4-PH alpha-2);

Alternative:

Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI).

Function:

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Subcellular Location:

Endoplasmic reticulum lumen

Protein Attributes:

Sequence length:

537

Sequence:

50:

MKLQVLVLVL | LMSWFGVLSW | VQAEFFTSIG | HMTDLIYAEK | DLVQSLKEYI |

100:

LVEEAKLAKI | KSWASKMEAL | TSRSAADPEG | YLAHPVNAYK | LVKRLNTDWP |

150:

ALGDLVLQDA | SAGFVANLSV | QRQFFPTDED | ESGAARALMR | LQDTYKLDPD |

200:

TISRGELPGT | KYQAMLSVDD | CFGLGRSAYN | EGDYYHTVLW | MEQVLKQLDA |

250:

GEEATVTKSL | VLDYLSYAVF | QLGDLHRAVE | LTRRLLSLDP | SHERAGGNLR |

300:

YFERLLEEER | GKSLSNQTDA | GLATQENLYE | RPTDYLPERD | VYESLCRGEG |

350:

VKLTPRRQKK | LFCRYHHGNR | VPQLLIAPFK | EEDEWDSPHI | VRYYDVMSDE |

400:

EIERIKEIAK | PKLARATVRD | PKTGVLTVAS | YRVSKSSWLE | EDDDPVVARV |

450:

NRRMQHITGL | TVKTAELLQV | ANYGMGGQYE | PHFDFSRSDD | EDAFKRLGTG |

500:

NRVATFLNYM | SDVEAGGATV | FPDLGAAIWP | KKGTAVFWYN | LLRSGEGDYR |

537:

TRHAACPVLV | GCKWVSNKWF | HERGQEFLRP | CGTTEVD

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Pfam:

KEGG:

SMR:

String:

Uniprot:

FOR

ELISA Kit for Mouse 4-PH alpha-2

Cat.:

E10872m

Price:

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MSDS:

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Packing:

96T

ELISA Kit for Mouse 4-PH alpha-2

MSDS:

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ELISA Kit for Mouse 4-PH alpha-2

Cat.:

E10872h

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Manual:

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Packing:

96T

Range:

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CLIA Kit for Mouse 4-PH alpha-2

Cat.:

U10872h

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MSDS:

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96T

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CLIA Kit for Mouse 4-PH alpha-2

MSDS:

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CLIA Kit for Mouse 4-PH alpha-2

Cat.:

U10872m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Mouse 4-PH alpha-2

Polyclonal Antibody for Mouse 4-PH alpha-2

Cat.:

P10872Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse 4-PH alpha-2

Monoclonal Antibody for Mouse 4-PH alpha-2

Monoclonal Antibody for Mouse 4-PH alpha-2

Monoclonal Antibody for Mouse 4-PH alpha-2

Protein for Mouse 4-PH alpha-2

Protein for Mouse 4-PH alpha-2

Protein for Mouse 4-PH alpha-2

R&D Technical Data

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Cloning, baculovirus expression, and characterization of a second mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta subunit."
Helaakoski T. , Annunen P. , Vuori K. , Macneil I.A. , Pihlajaniemi T. , Kivirikko K.I.
Proc. Natl. Acad. Sci. U.S.A.92:4427-4431(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB)
2.

"Characterization of the human and mouse genes for the alpha subunit of type II prolyl 4-hydroxylase. Identification of a previously unknown alternatively spliced exon and its expression in various tissues."
Nokelainen M. , Nissi R. , Kukkola L. , Helaakoski T. , Myllyharju J.
Eur. J. Biochem.268:5300-5309(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS IIA AND IIB)
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IIA)
4.

"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-482;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic fibroblast.

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